purified virus

The virus is purified by differential and rate-zonal centrifugation.

以差速离心和蔗糖密度梯度离心提纯了该病毒，纯化病毒的A260/A280为1.26；

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The virus was purified by means of chloroform clarification, polyethyleneglycol precipitation and differential centrifugation.

用氯仿澄清, 聚乙二醇沉淀和差速离心可部分提纯.

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Results: The gel filtration analysis of purified virus showed only one absorbance peak;

组织培养技术检测产物的生物学活性并计算纯化得率。结果：纯化后的病毒经凝胶过滤层析分析仅见一个层析峰；

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To the potyvirus group. The sap from infected tissue and purified virus preparation strongly reacts with antiserum against zucchini yellow mosaic virus ( ZYMV) in SDS-agar gel double diffusion tests.

染病组织粗汁液和提纯病毒制备物在SDS双向免疫扩散试验中与小西葫芦黄花叶病毒抗血清发生强烈的免疫沉淀反应；

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Recently we have prepared large amount of purified pEH 920 DNA which is inserted by complementary DNA of dengue virus 2 RNA.

新近制备了大量纯化的pEH920DNA，该质粒DNA插入了登革病毒2型核酸片段的互补DNA。

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The results of the quality analysis of the two different purified virus-containing fluids show that two different purified virus-containing fluids qualities all meet the specification of WHO and the two purified methods may all be used in preparation influenza vaccine.

对两种不同纯化方法得到的病毒原液进行质量分析，结果表明：用两种方法纯化后的病毒液质量都达到WHO规定的要求。两者均可作为流感疫苗（Influenza Vaccine）的纯化方法。

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The purified virus particles observed by electronic microscope were typical globosity with the diameter of about 20~ 30nm.

电镜观察病毒粒体为典型球状，直径约20～30nm。

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The purified virus preparation showed the ultraviolet absorption curve typical for filamentous virus and showed the following characteristics: OD. 260/ OD. 280 radio of 1.20, max.

紫外吸收光谱下为典型的线状病毒的曲线，并具有以下特点：OD260/OD280比值为1.20，max。

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The purified virus sample gained by sucrose density gradient centrifugation was proved to be typical absorbance curve of virus nucleoprotein and pure virus by methods of SDS-PAGE and electron microscopy and so on.

通过差速离心法和蔗糖密度梯度超速离心后所得的提纯病毒样品，用紫外分光光度法检测可见典型的病毒核蛋白吸收曲线，用SDS-PAGE分析和电镜观察也证明该样品为病毒纯品。

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